anti neun rabbit polyclonal antibody  (Proteintech)

Journal: Frontiers in Medicine

Article Title: Gastrodia elata fermentation alleviates methamphetamine-induced neuroinflammation and anxiety- and depression-like behaviors by regulating the PI3K-AKT signaling pathway

doi: 10.3389/fmed.2026.1768944

Figure Lengend Snippet: FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.

Article Snippet: SP Rabbit/Mouse HRP Kit (DAB) (Cat# SA00001-2/SA00001-1, CWBIO, China); DAB Substrate Kit (Cat# ZLI-9018, Zhongshan Jinqiao, Beijing, China); SPARKscript II RT Kit/SYBR Green RT-PCR Kit (Cat# AG0305-B/AH0104-B, SparkJade, China); Anti-Iba1 Rabbit Polyclonal Antibody (Cat# F155306, Abways, China); Anti-GFAP Rabbit Polyclonal Antibody (Cat# CY5424, Abcam, United Kingdom); Anti-NeuN Rabbit Polyclonal Antibody (Cat# 26975-1-AP, Proteintech, China); AKT/phosphorylated AKT (p-AKT)/phosphorylated PI3K (p-PI3K)/GAPDH (Cat# 10176-2-AP/66444-1-IG/20584-1-AP/60004-1-IG, Proteintech, China); PI3K (Cat# AF3242, Affinity, China).

Techniques: Immunohistochemical staining, Expressing

Journal: Frontiers in Oncology

Article Title: ASPHD1 Is a tumor-suppressive and prognostic marker in glioma

doi: 10.3389/fonc.2025.1694116

Figure Lengend Snippet: ASPHD1 overexpression induces neuronal differentiation and morphological changes in glioma cells. (A) Representative phase-contrast images of U87 cells transduced with vector control or ASPHD1-overexpressing (OE) plasmids. ASPHD1 OE cells exhibit more elongated and polarized morphology. Scale bar, 50 μm. (B) Quantification of cell aspect ratio in U87 cells (n=10 fields per group). Data are presented as mean ± SD. ****p < 0.0001 by unpaired two-tailed Student's t-test. (C–H) Correlation analyses between ASPHD1 and neuronal differentiation markers (ENO2, MAP2, TUBB3, RBFOX3, SYP and NeuroD1) in TCGA glioma cohorts. Scatter plots depict Pearson correlation coefficients and significance levels. (I, J) qPCR analysis of relative mRNA expression levels of stem cell (NESTIN, SOX2), oligodendrocyte (OLIG2, GALC), astroglial (S100B, GFAP), and neuronal (MAP2, TUBB3, RBFOX3, SYP) markers in U251 (I) and U87 (J) cells transduced with control or ASPHD1 OE vectors. Western blot analysis of NeuN expression in U251 (K) and U87 (L) cells following ASPHD1 overexpression (OE) or vector control transfection. Histone H3 (H3) was used as a loading control. Quantification of NeuN band intensity is shown on the right. Data represent mean ± SD (n=3). ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by unpaired two-tailed Student's t-test.

Article Snippet: The sheet was then incubated with primary antibodies against ASPHD1 (1:1000, sourced from Abcam, ab197301), NeuN rabbit polyclonal antibody (1:1000, provided by CST, #24307), Histone H3 rabbit polyclonal antibody (1:3000, provided by CST, ABL1141) and anti-Na + -K + -ATPase α1 rabbit polyclonal antibody (1:1000, provided by Abbkine, #4499).

Techniques: Over Expression, Transduction, Plasmid Preparation, Control, Two Tailed Test, Expressing, Western Blot, Transfection

Journal: Experimental Biology and Medicine

Article Title: Curcumin-enhanced elvitegravir therapy mitigates neuroinflammation and cognitive deficits in EcoHIV mice

doi: 10.3389/ebm.2025.10758

Figure Lengend Snippet: Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers NeuN, TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.

Article Snippet: Primary antibody incubation was carried out overnight at 4 °C using the following antibodies: NeuN rabbit polyclonal antibody (1:1000, Proteintech, Cat# 26975-1-AP), synaptophysin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 67864-1-Ig), GFAP rabbit polyclonal antibody (1:1000), L1CAM rabbit polyclonal antibody (Proteintech, Cat# 20659-1-AP), and β-actin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 66009-1-Ig) as an internal loading control.

Techniques: Infection, Western Blot, Expressing